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This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein.The second edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester,

Recombinant DNA --- Genetic engineering --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- rDNA --- DNA --- Genetic vectors

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This Series represents a compilation of the science-based consensus documents developed by the OECD Working Group on the Harmonisation of Regulatory Oversight in Biotechnology since 1995. The consensus documents are prepared by authorities of OECD members and other economies associated with this work. They contain information for use during the regulatory assessment of organisms produced from modern biotechnology - transgenic crops, trees, animals and micro-organisms - intended for release in the environment for agriculture, animal farming, forestry or other purpo650ses. Information relevant to environmental risk assessment (biosafety) includes biology, reproduction, genetics, ecology, and other elements. Knowledge of the traits introduced in the organisms, and their biotechnological developments, is also critical. These documents are of value to applicants for commercial uses of transgenic organisms, to regulators and risk assessors in charge of granting approvals to their environmental release, as well as to the wider scientific community.

Biotechnology industries --- Transgenic organisms --- Genetic engineering --- Risk assessment --- Genetically engineered organisms --- Genetically modified organisms --- GEOs (Genetically engineered organisms) --- GMOs (Genetically modified organisms) --- Organisms --- Biomedical industries --- High technology industries --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology

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"Tariffs for industrial products are a key element of the ongoing WTO negotiations. However, rather than clarifying the issues, the framework text agreed on 1 August 2004 leaves considerable uncertainty about the future direction of the talks. According to one view, the negotiations are back at first base, with little progress in evidence since the Fifth WTO Ministerial Conference, held in Cancún. Others see the texts as the basis for an ambitious approach to tariff cutting. The more ambitious proposals imply increased imports, lower tariff revenues, some labor market adjustments and reduced output in some key sectors in some developing regions. Furthermore, the main proposals do not fully resolve problems of tariff escalation and peaks. Proposals that take greater account of the need for special and differential treatment for developing countries seem less threatening and more likely to satisfy the wishes of the growing number of WTO members from developing countries. A successful outcome requires that the main focus be on high tariffs and market entry conditions in respect of products of export interest to developing countries. In addition, some way needs be found to assist some developing countries in coping with the likely adjustment costs of liberalization."--Publisher's description

Tariff --- Free trade --- 330.35 --- 338 --- 575.08:577.21 --- BPB0605 --- Economische groei. Kwantitatieve toename. Technische vooruitgang --zie ook {338.09} --- Economische situatie. Economische structuur van bepaalde landen en gebieden. Economische geografie. Economische produktie.economische produkten. Economische diensten --- Genetic engineering, genetic manipulation, recombinant DNA --- 575.08:577.21 Genetic engineering, genetic manipulation, recombinant DNA --- 338 Economische situatie. Economische structuur van bepaalde landen en gebieden. Economische geografie. Economische produktie.economische produkten. Economische diensten --- 330.35 Economische groei. Kwantitatieve toename. Technische vooruitgang --zie ook {338.09} --- Ad valorem tariff --- Border taxes --- Customs (Tariff) --- Customs duties --- Duties --- Fees, Import --- Import controls --- Import fees --- Tariff on raw materials --- Commercial policy --- Indirect taxation --- Revenue --- Customs administration --- Favored nation clause --- Non-tariff trade barriers --- Reciprocity (Commerce) --- Free trade and protection --- Trade, Free --- Trade liberalization --- International trade

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A continuous development in plant biotechnology including gene technology has been observable during the past 20 years. Different methods elaborated with model plants were also applied to forest trees on a larger scale. Whereas in the beginning the meaning of the term “plant biotechnology” embraced a wide variety of meanings like, e. g. , regeneration of plantlets via tissue culture, embryo rescue, somatic embryogenesis and gene transfer, the focus of this term has changed more and more. Nowadays, it is the transfer of genes which comes into mind when plant biotechnology is discussed, including of course the evaluation of all challenges and risks related to gene transfer methods. Compared with annual plants, especially in the field of agriculture, the work and the progress with transgenic trees is still in its infancy. Nevertheless, but often unnoticed by the scientific community, there are a few countries which already allow the commercial use of a restricted number of transgenic tree clones after different critical steps of approval. This and the ongoing improvement in transgenic research in trees led to the idea of preparing a summary of the present state of the art from different points of view. With the help of a number of authors directly or indirectly involved in tree transgenesis, this book was produced.

Trees --- Transgenic plants. --- Genetic engineering. --- GE crops (Genetically engineered crops) --- GE plants (Genetically engineered plants) --- Genetically engineered crops --- Genetically engineered plants --- Genetically modified crops --- Genetically modified plants --- GM crops (Genetically modified crops) --- GM plants (Genetically modified plants) --- Novel crops --- Transgenic crops --- Plants, Cultivated --- Transgenic organisms --- Plant genetic engineering --- Dendrology --- Nursery stock --- Woody plants --- Arboriculture --- Forests and forestry --- Timber --- Trees. --- Plant genetics. --- Plant Ecology. --- Plant physiology. --- Environmental toxicology. --- Tree Biology. --- Plant Genetics and Genomics. --- Genetic Engineering. --- Plant Physiology. --- Ecotoxicology. --- Ecotoxicology --- Pollutants --- Pollution --- Environmental health --- Toxicology --- Botany --- Plants --- Physiology --- Ecology --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Genetics --- Phytoecology --- Vegetation ecology --- Plant ecology. --- Floristic ecology

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Genetic Engineering: Principles and Methods presents state-of-the-art discussions in modern genetics and genetic engineering. Recent volumes have covered gene therapy research, genetic mapping, plant science and technology, transport protein biochemistry, and viral vectors in gene therapy, among many other topics. Volume Key Features: Genetic Engineering: Principles and Methods, Volume 27 contains discussions of contemporary and relevant topics in genetics, including: Identification and Analysis of Micrornas Dormancy and the Cell Cycle Long distance peptide and metal transport in plants Signaling in plant response to temperature and water stresses Nutrient transport and metabolism in plants Salt Stress Signaling and Mechanisms of Plant Salt Tolerance Gene cloning and expression Assisted folding and assembly of proteins This principles and methods approach to genetics and genetic engineering is essential reading for all academics, bench scientists, and industry professionals wishing to take advantage of the latest and greatest in this continuously emerging field. Jane K. Setlow, PhD. has championed the field of genetics for over 30 years, agreeing to edit Genetic Engineering: Principles & Methods since its inception in 1979. An internationally recognized scientist with numerous publications, Dr. Setlow is the former Chairperson of the NIH Recombinant DNA Molecule Advisory Committee (RAC). Her lab can found at Brookhaven National Laboratory, Upton, New York.

Clone Cells. --- DNA. --- Genetic Engineering. --- Genetic engineering --- Cloning --- DNA --- Génie génétique --- Clonage --- ADN --- EPUB-LIV-FT SPRINGER-B LIVBIOMO LIVMEDEC --- Genetic engineering. --- Genetic recombination. --- Designed genetic change --- Engineering, Genetic --- Gene splicing --- Genetic intervention --- Genetic surgery --- Genetic recombination --- Biotechnology --- Transgenic organisms --- Recombination, Genetic --- Chromosomes --- Recombinant DNA --- Human genetics. --- Biochemistry. --- Botany. --- Human Genetics. --- Biochemistry, general. --- Plant Sciences. --- Botanical science --- Phytobiology --- Phytography --- Phytology --- Plant biology --- Plant science --- Biology --- Natural history --- Plants --- Biological chemistry --- Chemical composition of organisms --- Organisms --- Physiological chemistry --- Chemistry --- Medical sciences --- Genetics --- Heredity, Human --- Human biology --- Physical anthropology --- Composition --- Plant science. --- Floristic botany